Expression of green fluorescent protein in Xylella fastidiosa is affected by passage through host plants.
Identifieur interne : 000916 ( Main/Exploration ); précédent : 000915; suivant : 000917Expression of green fluorescent protein in Xylella fastidiosa is affected by passage through host plants.
Auteurs : Xiaoting Qin [États-Unis] ; John S. HartungSource :
- Current microbiology [ 0343-8651 ] ; 2004.
Descripteurs français
- KwdFr :
- Citrus (microbiologie), Conjugaison génétique (MeSH), Expression des gènes (MeSH), Fluorescence (MeSH), Maladies des plantes (microbiologie), Protéines luminescentes (génétique), Protéines recombinantes (génétique), Protéines à fluorescence verte (MeSH), Vitis (microbiologie), Xylella (génétique), Xylella (pathogénicité), Xylella (physiologie).
- MESH :
- génétique : Protéines luminescentes, Protéines recombinantes, Xylella.
- microbiologie : Citrus, Maladies des plantes, Vitis.
- pathogénicité : Xylella.
- physiologie : Xylella.
- Conjugaison génétique, Expression des gènes, Fluorescence, Protéines à fluorescence verte.
English descriptors
- KwdEn :
- Citrus (microbiology), Conjugation, Genetic (MeSH), Fluorescence (MeSH), Gene Expression (MeSH), Green Fluorescent Proteins (MeSH), Luminescent Proteins (genetics), Plant Diseases (microbiology), Recombinant Proteins (genetics), Vitis (microbiology), Xylella (genetics), Xylella (pathogenicity), Xylella (physiology).
- MESH :
- chemical , genetics : Luminescent Proteins, Recombinant Proteins.
- chemical : Green Fluorescent Proteins.
- genetics : Xylella.
- microbiology : Citrus, Plant Diseases, Vitis.
- pathogenicity : Xylella.
- physiology : Xylella.
- Conjugation, Genetic, Fluorescence, Gene Expression.
Abstract
Xylella fastidiosa, a Gram-negative bacterial plant pathogen, causes Pierce's disease of grapevine in North America. In South America the pathogen causes citrus variegated chlorosis, which is widespread in Brazil. We have introduced into Xylella fastidiosa a mini-Tn5 transposon that encodes a green fluorescent protein (GFP) gene optimized for expression in bacteria. The mini-Tn5 derivative was inserted into different sites of the genome in independent transconjugants as determined by Southern blotting. The GFP gene was expressed well and to different levels in different transconjugants. Four independent transconjugants were separately used to inoculate sweet orange and tobacco seedlings. The transconjugants were able to colonize the plants and were subsequently isolated from points distal to the inoculation sites. When the relative fluorescence of the transconjugants that had been passed through either tobacco or sweet orange was compared with that of the same transconjugant maintained continuously in vitro, we observed that passage through either plant host significantly increased the level of expression of the GFP. The increased level of expression of GFP was transient, and was lost upon further culture in vitro. Xylella fastidiosa forms biofilms in planta which are believed to represent a metabolically differentiated state. The increased expression of GFP observed after passage through plants may be accounted for by this phenomenon.
DOI: 10.1007/s00284-004-4345-0
PubMed: 15386107
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
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In South America the pathogen causes citrus variegated chlorosis, which is widespread in Brazil. We have introduced into Xylella fastidiosa a mini-Tn5 transposon that encodes a green fluorescent protein (GFP) gene optimized for expression in bacteria. The mini-Tn5 derivative was inserted into different sites of the genome in independent transconjugants as determined by Southern blotting. The GFP gene was expressed well and to different levels in different transconjugants. Four independent transconjugants were separately used to inoculate sweet orange and tobacco seedlings. The transconjugants were able to colonize the plants and were subsequently isolated from points distal to the inoculation sites. When the relative fluorescence of the transconjugants that had been passed through either tobacco or sweet orange was compared with that of the same transconjugant maintained continuously in vitro, we observed that passage through either plant host significantly increased the level of expression of the GFP. The increased level of expression of GFP was transient, and was lost upon further culture in vitro. Xylella fastidiosa forms biofilms in planta which are believed to represent a metabolically differentiated state. The increased expression of GFP observed after passage through plants may be accounted for by this phenomenon.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">15386107</PMID><DateCompleted><Year>2004</Year><Month>11</Month><Day>04</Day></DateCompleted><DateRevised><Year>2004</Year><Month>12</Month><Day>15</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0343-8651</ISSN><JournalIssue CitedMedium="Print"><Volume>49</Volume><Issue>3</Issue><PubDate><Year>2004</Year><Month>Sep</Month></PubDate></JournalIssue><Title>Current microbiology</Title><ISOAbbreviation>Curr Microbiol</ISOAbbreviation></Journal><ArticleTitle>Expression of green fluorescent protein in Xylella fastidiosa is affected by passage through host plants.</ArticleTitle><Pagination><MedlinePgn>215-20</MedlinePgn></Pagination><Abstract><AbstractText>Xylella fastidiosa, a Gram-negative bacterial plant pathogen, causes Pierce's disease of grapevine in North America. In South America the pathogen causes citrus variegated chlorosis, which is widespread in Brazil. We have introduced into Xylella fastidiosa a mini-Tn5 transposon that encodes a green fluorescent protein (GFP) gene optimized for expression in bacteria. The mini-Tn5 derivative was inserted into different sites of the genome in independent transconjugants as determined by Southern blotting. The GFP gene was expressed well and to different levels in different transconjugants. Four independent transconjugants were separately used to inoculate sweet orange and tobacco seedlings. The transconjugants were able to colonize the plants and were subsequently isolated from points distal to the inoculation sites. When the relative fluorescence of the transconjugants that had been passed through either tobacco or sweet orange was compared with that of the same transconjugant maintained continuously in vitro, we observed that passage through either plant host significantly increased the level of expression of the GFP. The increased level of expression of GFP was transient, and was lost upon further culture in vitro. Xylella fastidiosa forms biofilms in planta which are believed to represent a metabolically differentiated state. The increased expression of GFP observed after passage through plants may be accounted for by this phenomenon.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Qin</LastName><ForeName>Xiaoting</ForeName><Initials>X</Initials><AffiliationInfo><Affiliation>United States Department of Agriculture, Agricultural Research Service, Fruit Laboratory, 10300 Baltimore Avenue, Beltsville, Maryland, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Hartung</LastName><ForeName>John S</ForeName><Initials>JS</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Curr Microbiol</MedlineTA><NlmUniqueID>7808448</NlmUniqueID><ISSNLinking>0343-8651</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D008164">Luminescent Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D011994">Recombinant Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>147336-22-9</RegistryNumber><NameOfSubstance UI="D049452">Green Fluorescent Proteins</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D002957" MajorTopicYN="N">Citrus</DescriptorName><QualifierName UI="Q000382" MajorTopicYN="Y">microbiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D003227" MajorTopicYN="N">Conjugation, Genetic</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D005453" MajorTopicYN="N">Fluorescence</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D015870" MajorTopicYN="N">Gene Expression</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D049452" MajorTopicYN="N">Green Fluorescent Proteins</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008164" MajorTopicYN="N">Luminescent Proteins</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010935" MajorTopicYN="N">Plant Diseases</DescriptorName><QualifierName UI="Q000382" MajorTopicYN="N">microbiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D011994" MajorTopicYN="N">Recombinant Proteins</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D027843" MajorTopicYN="N">Vitis</DescriptorName><QualifierName UI="Q000382" MajorTopicYN="Y">microbiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D044167" MajorTopicYN="N">Xylella</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000472" MajorTopicYN="Y">pathogenicity</QualifierName><QualifierName UI="Q000502" MajorTopicYN="N">physiology</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>2004</Year><Month>9</Month><Day>24</Day><Hour>5</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2004</Year><Month>11</Month><Day>5</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2004</Year><Month>9</Month><Day>24</Day><Hour>5</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">15386107</ArticleId><ArticleId IdType="doi">10.1007/s00284-004-4345-0</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>États-Unis</li></country><region><li>Maryland</li></region></list><tree><noCountry><name sortKey="Hartung, John S" sort="Hartung, John S" uniqKey="Hartung J" first="John S" last="Hartung">John S. Hartung</name></noCountry><country name="États-Unis"><region name="Maryland"><name sortKey="Qin, Xiaoting" sort="Qin, Xiaoting" uniqKey="Qin X" first="Xiaoting" last="Qin">Xiaoting Qin</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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